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. 2022 Jun 26;117(3):219–234. doi: 10.1080/20477724.2022.2088496

Table 4.

Kinetic values of OXA-10 β-lactamase against β-lactam antibiotics.

  kcat (s−1) Km (µM) kcat/Km (mM−1·s−1)
Carbapenems
Imipenem <0.1 [62]
0.041 ± 0.001 [30]
0.047 ± 0.001 [31]		 0.04 ± 0.007 [62]
2.0 ± 0.1 [30]
>2 [31]		 ND [62]
21 ± 3 [30]
>23 [31]
Meropenem 0.039 ± 0.001 [30]
0.023 ± 0.001[31]		 5.6 ± 0.8[30]
>2 [31]		 7 ± 0.2 [30]
>12 [31]
Ertapenem 0.022 ± 0.001 [30] 4.1 ± 0.5 [30] 5.4 ± 0.7 [30]
Doripenem 0.037 ± 0.001 [30] 4.8 ± 0.8 [30] 8 ± 1 [30]
Penicillins
Benxylpenicillin
(Penicillin G)		 89 ± 10 [60]
109 ± 3 [16]
120 ± 10 [104]
120 ± 5 [105]
91 ± 1 [31]		 63 ± 6 [60]
23 ± 0.4 [16]
20 ± 1 [104]
20 ± 1 [105]
14 ± 1 [31]		 1.41x103 [60]
(5 ± 1)x103 [16]
(6.0 ± 0.6)x103 [104]
(6 ± 0.4)x103 [105]
6.7x103 [31]
Oxacillin 608 ± 10 [60]
(1.26 ± 0.03)x103 [16]
300 ± 10 [104]
300 ± 4 [105]
660 [62]
530 ± 10 [30]
346 ± 3 [31]		 222 ± 16 [60]
29 ± 2 [16]
100 ± 20 [104]
100 ± 20 [105]
96 [62]
87 ± 5 [30]
65 ± 5 [31]		 2.74x103 [60]
(43 ± 3)x103 [16]
(3 ± 0.6)x103 [104]
(3 ± 0.6)x103 [105]
6.9x103 [62]
(6.1 ± 0.3)x103 [30]
5.3x103 [31]
Ampicillin (5.59 ± 0.03)x103 [60]
143 ± 7 [16]
220 ± 20 [104]
220 ± 10 [105]
530 [62]		 235 ± 30 [60]
34 ± 4 [16]
35 ± 5 [104]
35 ± 5 [105]
77 [62]		 2.5x103 [60]
(4.2 ± 0.6)x103 [16]
(6.02 ± 0.1)x103 [104]
(6.3 ± 0.9)x103 [105]
6.9x103 [62]
Carbenicillin 31 ± 1 [60]
112 ± 14 [16]		 195 ± 13 [60]
92 ± 16 [16]		 159 [60]
(1.2 ± 0.3)x103 [16]
Cloxacillin 530 ± 36 [60]
(1.53 ± 0.02)x103 [16]
120 ± 10 [104]		 (2.64 ± 0.3)x103 [60]
114 ± 22 [16]
110 ± 10 [104]		 196 [60]
(13 ± 3)x103 [16]
(1 ± 0.1)x103 [104]
Cephalosporins
Cephalothin 6 ± 0.1 [60]
8.3 ± 0.1 [16]
2.5 ± 0.1 [104]
10 ± 1 [105]
2.9 ± 0.1 [62]
1.07 ± 0.03 [31]		 38 ± 2 [60]
32 ± 2 [16]
7 ± 1 [104]
35 ± 2 [105]
11 ± 1.4 [62]
4.4 ± 1.4 [31]		 158 [60]
260 ± 10 [16]
380 ± 50 [104]
300 ± 30 [105]
260 [62]
250 [31]
Cephaloridine 79 ± 8 [60]
57 ± 14 [16]
70 ± 20 [104]		 (2.34 ± 0.3)x103 [60]
374 ± 94 [16]
400 ± 100 [104]		 33 [60]
150 ± 50 [16]
180 ± 60 [104]
Cefoxitin >0.07 [31] >200 [31] 0.38 ± 0.012 [31]
Cefotaxime 9 ± 0.2 [60]
2.06 ± 0.19 [31]		 346 ± 19 [60]
104 ± 17 [31]		 26 [60]
19 [31]
Ceftriaxone 3 ± 0.3 [60] 55 ± 2 [60] 54 [60]
Ceftazidime ND [60] ND [60] ND [60]

ND: Hydrolysis not detected.