Figure 1:

Prophylactic treatment with PEPITEM reduced leukocyte trafficking induced by 6 weeks of a high-fat obesogenic diet. (A) Schematic representation of experimental time course where wild-type male mice were prophylactically implanted with a mini pump, which continuously released 0.0822 mg/week of PEPITEM or PBS (as a control), and fed HFD for 6 weeks. (B) Representative images of pancreatic islets from PBS or PEPITEM-treated mice were stained with insulin-producing beta cells in red and glucagon-releasing alpha cells in green and imaged (scale bar = mm²) (for colour figure refer to online version). White box represents a single pancreatic islet shown at increased magnification (scale bar = µm²). Images were quantified for (C) the number of the islets/10 000 µm² of whole area and (D) the area of islets/10 000 µm² of whole area. (E) visceral adipose tissue CD3⁺ T-cells, (F) visceral adipose tissue CD4⁺ T cells, (G) visceral adipose tissue CD3⁺KLRG1⁺ T-cells, (H) peritoneal lavage CD3⁺ T cells, (I) peritoneal lavage CD3⁺KLRG1⁺ T cells and (J) peritoneal lavage F4/80^HCD11c^Int macrophages were quantified using flow cytometry analysis. Absolute number of immune cells were normalized to bead counts and plotted as number per g of adipose tissue and per ml of peritoneal lavage. Data are mean ± SEM using n = 5 mice per group from n = 1 independent experiment. *P < 0.05, **P < 0.01, and ***P < 0.001 by unpaired t-test compared to PBS treated.