Figure 2:

Therapeutic treatment with PEPITEM altered the patterns of leukocyte trafficking into peripheral tissues induced by 12 weeks of a high-fat obesogenic diet. (A) Schematic representation of experimental time course where mice were fed HFD for 12 weeks. At week 6, mice were implanted with a mini pump to allow therapeutic continuous release 0.0822 mg/week of PEPITEM or PBS (as a control) for the last 6 weeks of the HFD. Pancreatic islets from PBS or PEPITEM-treated mice were stained insulin-producing beta cells and glucagon-releasing alpha cells. Images quantified for (B) the number of the islets/10 000 µm² of whole area and (C) the area of islets/10 000 µm² of whole area. (D) Visceral adipose tissue CD3⁺ T cells, (E) visceral adipose tissue CD4⁺ T cells, (F) visceral adipose tissue CD3⁺KLRG1⁺ T-cells, (G) peritoneal lavage F4/80^HCD11c^Int macrophages, (H) peritoneal lavage CD3⁺KLRG1⁺ T cells, (I) blood CD3⁺KLRG1⁺ T-cells, (J) blood CD19⁺CD43⁺CD93⁺CD23^−- CD21⁻ age-associated B-cells, and (K) blood LyG6⁺ neutrophils were quantified using flow cytometry analysis. Absolute number of immune cells were normalized to bead counts and plotted as number per g of adipose tissue and per ml of peritoneal lavage or blood. Data are mean ± SEM using n = 14 mice per group from n = 1 independent experiment. *P < 0.05 and **P < 0.01 by unpaired t-test compared to PBS treated.