Figure 3:

Therapeutic treatment with PEPITEM altered leukocyte trafficking into secondary lymphoid tissues in obese mice. Mice were fed HFD for 12 weeks and received 0.0822 mg/week of PEPITEM or PBS as a control for the last 6 weeks of the HFD for the full duration. (A) Splenic CD45⁺ T cells, (B) splenic CD3⁺ T cells, (C) inguinal lymph node CD45⁺ T cells, (D) inguinal lymph node CD3⁺ T cells, (E) splenic CD19⁺ B cells, and (F) splenic age-associated B-cells were quantified using flow cytometry analysis. Absolute number of immune cells were normalized to bead counts and plotted as number per g of tissue. Data are mean ± SEM using n = 14 mice per group from n = 1 independent experiment. *P < 0.05 and **P < 0.01 by unpaired t-test compared to PBS treated.