Figure 1. Suspended drop crystallization.

(a) A support-free EM grid is clipped into an autogrid cartridge and mounted between the arms of the suspended drop screening tool. The sample and crystallization solution are dispensed onto the grid. (b) The chamber is immediately sealed to allow vapor diffusion. (b) The incubation chambers are inserted into a screening tray for efficient storing and monitoring of crystallization progress by light, fluorescence and UV microscopy. (d) EM grids containing crystals are retrieved from the screening tool and frozen. (e) The specimen is then interrogated by MicroED or other methods such as tomography, x-ray crystallography, or general microscopy. FIB milling is optional depending on the application.