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[Preprint]. 2023 Mar 29:rs.3.rs-2631490. [Version 1] doi: 10.21203/rs.3.rs-2631490/v1

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Experimental design, implementation, and automation of the skeletal muscle MPS payload for the International Space Station. a Human cell banks. Skeletal muscle biopsies were obtained from the vastus lateralis from volunteers (AdventHealth, Orlando). Isolated muscle precursor cultures were enriched for CD56+ (myogenic) cells. b Experimental flight timeline. Tissue chips were seeded with myoblasts pre-differentiated, loaded into the CubeLab^™ and launched to the ISS on SpaceX CRS-21. Crew members installed the PAUL on the EXPRESS rack locker and the on-orbit experiment was initiated after plug-in. 10 days post launch, crew members, moved the payload to cold stowage following experiment termination with RNALater. c On-orbit real-time telemetry. Recording of YA-derived myobundle during electrical stimulation on-orbit and resultingdominant frequency of contraction determined by Fast Fourier Transform of the time series signal of displacement.