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. 2023 Apr 7;13:5748. doi: 10.1038/s41598-023-32957-y

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Establishment of Crispr-Cas12a fluorescence detection system. (a) Schematic diagram of the Crispr for IDH1-R132H detection. Nucleic acid extraction, detection, result determination and reporting were all integrated in a single instrument, the whole process could obtain test results in 60 min. (b) The Crispr detection system for IDH1-R132H. The change of the fluorescence value was recorded at the end of the reaction, and the IDH1-R132H was determined according to the threshold of the fluorescence value (link: https://www.adobe.com/products/illustrator.html/, version 24.0.1).