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. 2023 Apr 7;13:5748. doi: 10.1038/s41598-023-32957-y

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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ROC curve of Crispr method. (a) ROC curve in Group 1. The value of AUC was 0.939 (95% CI 0.900–0.975); the threshold of relative fluorescence value was 425; the sensitivity, specificity and consistency rate was 91.4%, 95.7% and 93.1%, respectively. (b) ROC curve in Group 2. The value of AUC was 0.938 (95% CI 0.893–0.983); the threshold of relative fluorescence value was 525; the sensitivity, specificity and consistency rate was 96.1%, 89.7% and 92.0%, respectively (link: https://www.originlab.com/, version OriginPro 2018C).