Table 3.
The fluorescence detection system of Crispr-Cas12a.
| Subjects | Volume |
|---|---|
| Fluorescence probe | 50 pm |
| Target DNA template | 1–5 μL |
| crRNA | 0.75 µM |
| FnCas12a | 1.5 µM |
| NEBuffer2.1 | 3 μL |
| DEPC-water | 100 μL |
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