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. 2023 Apr 7;9(14):eadd4791. doi: 10.1126/sciadv.add4791

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Fig. 3. — Confocal micrograph showing the localization of the two PS biosensors 2PH^EVECTIN (A) and C2^LACT (C) along 1 μM RALF23 treatment and their respective quantification (B and D). (E) Confocal micrograph of fer-4xC2^LACT after RALF23 treatment and its PM/intracellular ratio quantification (F). (G) DHE fluorescence quantification of Col-0 plants in the presence or absence of osmotic stimulation (−0.75 MPa) with or without RALF23 pretreatment. Error bars correspond to the 95% confidence interval. According to ANOVA followed by Tukey test, letters indicate significant differences among means (P < 0.01). For (B) and (D), at 15 and 60 min, t test was used for comparison between control and RALF23 treated plants. * P < 0.05, n > 15 from three independent biological replicates. Scale bars, 10 μm.