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. 2023 Apr 7;9(14):eadd1581. doi: 10.1126/sciadv.add1581

Fig. 4. Analysis of collective migration.

Fig. 4.

(A and B) CL in the direction parallel to the topographic noise gradient (CLx) or orthogonal to it (CLy) for MDCK EGFP or HDF collectives. Error bars correspond to the measured standard deviation of the mean. (C) Velocity of the front edge for MDCK EGFP cells and HDF collectives on substrates featuring different levels of topographic noise. Error bars correspond to the measured standard error of the mean. (D) ΔMv/ΔITN% of the front edge migrating along the direction of increasing (+ΔITN%) or decreasing (−ΔITN%) topographic noise in the representative 0% ≤ ITN% ≤ 40% interval. Experiments performed on substrate B (table S1). Error bars correspond to the measured standard deviation of the mean. (E) Average number of leader cells as a function of the local level of topographic noise. *P < 0.05, **P < 0.01, and ***P < 0.001; ns, not significant, by two-sample t test and Mann-Whitney test. (F to H) Representative inverted fluorescent images of migratory edge depicting the evolution of the wound edge profile, for MDCK EGFP cells seeded on ITN% = 100, 0, or 50% regions. Scale bar, 200 μm. (I to K) Evolution of the migration velocity for cells at the front edge on the ITN% = 100, 0, or 50% regions. Results showing one representative experiment of n ≥ 3 and n′ ≥ 2. Error bars correspond to the measured standard deviation of the mean. n = number of independent experiments, n′ = number of analyzed fields of view.