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. 2023 Mar 31;2023:1230182. doi: 10.1155/2023/1230182

Figure 2.

Figure 2

SAV1 suppresses GC cell proliferation in vitro. (a) Transfection efficiency of SAV1 overexpression vectors and shRNA in GC cell lines determined via western blotting. AGS and NCI-N87 cells were transfected with pSAV1 or control vectors. HTB103 cells were transfected with shSAV1-1, shSAV1-2, or control shRNAs. AGS and NCI-N87 cells were infected with retroviruses containing SAV1 (pBABE-SAV1) and empty retroviral expression vector (pBABE-puro). (b, c) The colony formation assay was performed on AGS and HTB103 cells in 24-well plates, and the numbers of colonies were counted after 14 days. (d, e) The spheroid colony formation assay was performed on AGS and NCI-N87 cells. SAV1 overexpression decreased the number and size of the first and second generations of spheroids. SAV1, Salvador homologue 1; GC, gastric cancer; shRNA, short hairpin RNA.