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. Author manuscript; available in PMC: 2024 Apr 1.
Published in final edited form as: J Allergy Clin Immunol. 2022 Dec 5:S0091-6749(22)01622-0. doi: 10.1016/j.jaci.2022.11.016

Figure 7. LIGHT induces non-canonical NF-κB-dependent activation of Rac1/PAK1 and phosphorylation of MLC in ASM.

Figure 7.

(A-C) pMLC2 (A), pMYPT and pPAK1 (B), and active Rac1-GTP immunoprecipitated with PAK1 (C) in human ASM stimulated with LIGHT for the indicated times. (D-E) Processing of p52, and pPAK1 (D) and pMLC2 (E) in ASM stimulated with LIGHT for 12hr, with or without inhibitors of canonical (BAY) and non-canonical NF-κB (NIK-SMI). (F) Active Rac1-GTP immunoprecipitated with PAK1 in ASM stimulated with LIGHT for 4hr with or without NIK-SMI. All data in A-F representative of 3 experiments. (G) Gel contraction of ASM stimulated with LIGHT, with or without an inhibitor of Rac1. Percentage gel contraction compared to PBS in triplicates. (H) Confocal analysis of ASM treated with LIGHT with or without a Rac1 inhibitor. Volume of phalloidin and vinculin normalized to cell size from triplicates (scale 10 μm). 300–400 ASM, in two replicate experiments were analyzed. Data representative of 2 experiments. Data means ± SEM. **P < 0.01, ***P < 0.001, ****P < 0.0001.