Flow diagram of the changes in numbers of PE1, PE2, PE3, PE4 (and PE5) classes of predicted proteins from 2021–02 to 2022–02. Blue solid arrows indicate increases to PE1; red arrows indicate demotions from PE1 or PE2,3,4 MPs, to PE5; black arrows show increases to MPs. Dashed arrows indicate continuation in the same status. In the margins we show examples of the 12 new entries to and 32 removals from neXtProt (see discussion later about uORFs and smORFs). Note, PE5 represents a category of dubious or uncertain genes, mostly pseudogenes, which we have excluded from the HPP metrics since 2013.