Fig. 4. 10-NO₂-OA inhibits rotenone-induced LRRK2 activation in dopaminergic neurons of SNpc.

a PL assay for in situ detection of LRRK2 kinase activity (PL pS1292-LRRK2) (Cohort 2). Rotenone treatment elevated LRRK2 kinase activity (red), which was inhibited by treatment with 10-NO₂-OA (45 mg/Kg) in DA neurons of SNpc (TH, blue—Scale bar: 35 μm). b Quantification of the PL pS1292-LRRK2 fluorescence signal. Symbols represent the normalized means of the intensity (with Vehicle set at 100%) from a single rat (6 slices/brain). Statistical analysis was performed by one-way ANOVA with post hoc Bonferroni correction (*p < 0.0001 compared to Vehicle, ^#p < 0.0001 compared to Rotenone).