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. 2023 Apr 7;13:5729. doi: 10.1038/s41598-023-31352-x

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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The impact of pericytes on network stability in response to starvation. (A) 72 h nutrient starvation resulted in the entire collapse of HUVEC networks in monocultures. In contrast, HUVEC/ pericyte networks persisted, although they apparently regressed compared with untreated conditions (mean ± SEM total tube length, 10.7 ± 0.7 vs 13.5 ± 0.6 mm/field of view, respectively). (B) Impact of starvation on tube diameter in co-cultures, showing no significant difference after starvation. (C) Representative images, z-projection images were generated using confocal images. Red, CD31. Scale bar: 300 µm. N = 3; n.s., non significant; *, p < 0.05; ***, p < 0.001.