Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2023 Apr 7;8:21. doi: 10.1038/s41536-023-00293-4

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Fig. 5 — a Quantitative RT-PCR of kdm genes expression, normalized by gapdh. b–d Representative images of RNAscope in situ hybridization with kdrl and kdm7aa probes (scale bar, 100 μm) and high-magnification image of boxed region in (c) (arrowheads, double kdrl- and kdm7aa-positive endothelial cells). e Immunoprecipitation (IP) assays with either anti-Myc or anti-Brg1 antibody in 293T cells. Inputs were used as loading controls and IgG as negative controls. The blots were derived from the same experiment, and they were processed in parallel. f Quantitative RT-PCR analysis showing that the expression levels of notch1a, notch1b, notch2, and notch3 from hearts at 7 dpa injected with encapsulated kdm7aa siRNA was higher compared with the control siRNA group. Data represented one of three independent experiments. Data were mean fold changes ± s.e.m., ***p < 0.005, unpaired t-test. g Luciferase reporter assays in 293T cells stably expressing the notch promoter-luciferase reporter in the pGL4.26 vector. Expression plasmid clones containing kdm7aa, brg1, or dn-xbrg1 were co-transfected into cells stably expressing each notch reporter. Data represented one of two independent experiments, ***p < 0.005, one-way analysis of variance (F = 110.6 for the left panel and 60.3 for the right panel) followed by the Bonferroni test. n number shown here (f and g) indicated technical replicates. h–j Representative images of immunostaining showing the numbers of Mef2C⁺/PCNA⁺ proliferating cardiomyocytes (arrowheads, Mef2C⁺/PCNA⁺ proliferating cardiomyocytes; scale bar, 100 μm). k Statistics of panels (h–k) (n numbers indicated biological replicates; data were the mean ± s.e.m.; ***p < 0.005; one-way analysis of variance [F = 36.79] followed by Dunnett’s multiple comparison test).