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. 2023 Apr 7;6:378. doi: 10.1038/s42003-023-04761-x

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 4 — a Scatter pie plots displaying the cell-type proportion estimates of SpaDecon, RCTD, SPOTlight, Stereoscope, and cell2location at each spot, along with heatmaps showing the estimated distributions of malignant, B, and T cells. b Boxplot showing the proportions of malignant cells across stromal spots estimated by each method (n = 25 spots). c Manual annotation of histology image of melanoma tissue section from which ST data were obtained.