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. 2023 Apr 7;13:5708. doi: 10.1038/s41598-023-32955-0

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Comparison of the whole slide image focus quality by our developed scanner and Olympus VS120 scanner. (a1) Whole slide image (WSI) of a model sample under 20X objective from our developed scanner. (a2) WSI of the same model sample under 20X objective from Olympus VS120 scanner. (b1,b2) and (c1,c2) are the zoom-in on the same regions from two WSIs. Their area size is the same as the image tile from VS120 scanner, 366 μm × 287 μm. (d) Quantitative analysis of focus quality of WSI from both scanners in blue, green and red channel.