Table 1.

Biological samples used in the study

Genotypea				Origin
ITS	18S	cox1 mitotype	Genotypea	Laboratory/field isolate	Country	Life-cycle stage	n
Sh	Sh	Sh	Sh	Laboratory	Egypt	Adult	3
Sh	Sh	Sh	Sh	Field	Malic	Miracidia	58
Sb	Sb	Sb	Sb	Laboratory	Spain	Adult	3
Sb	Sb	Sb	Sb	Field	Mali	Adult	37
Sc	Sc	Sc	Sc	Field	Mali	Adult	31
Sc	Sc	Sc	Sc	Field	Senegal	Adult	12
Sh and (Sb or Scb)	Sh and Sb	Sb	Hybrid Sh/Sb	Laboratory F1	Egypt/Spain	Adult	3
Sh and (Sc or Sbb)	Sh and Sc	Sh	Hybrid Sh/Sc	Field	Malic	Miracidia	19
Sh and (Sc or Sbb)	Sh and Sc	Sh	Hybrid Sh/Sc	Field	Ivory Coastd	Miracidia	3

cox1, Cytochrome oxidase subunit 1 mitochondrial gene, ITS internal transcribed spacer, n number of individuals, Sb Schistosoma bovis, Sc Schistosoma curassoni, Sh Schistosoma haematobium, SNP single nucleotide polymorphism, T–ARMS tetra-primer amplification refractory mutation system

^aThe genetic profiles are inferred by Sanger sequencing of the T-ARMS regions of the ITS, 18S and by the rapid diagnostic multiplex PCR (RD-PCR) region of the cox1 DNA [45]

^bThe sequence of the ITS or 18S cannot be distinguished between the two species as the region analysed does not contain species-specific SNPs

^cEthical clearance was obtained from the Faculty of Medicine, Pharmacology, and Odonto-Stomatology of Mali (reference no.2018/71/CE/FMPOS)

^dEthical clearance was obtained from the Ministry of Health and Public Hygiene in Ivory Coast (reference no.003–18/MSHP/CNER-kp) [38]