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Primer pairs used for detection-resistant genes.
| Genes target | Sequences (5′–3′) class (Forward-reverse) | Annealing temperature (°C) | Amplicon size (bp) | References |
|---|---|---|---|---|
| tetA | GGTTCACTCGAACGACGTCA | 60 | 577 | [22] |
| CTGTCCGACAAGTTGCATGA | ||||
| tetB | TACGTGAATTTATTGCTTCGG | 60 | 206 | [23] |
| ATACAGCATCCAAAGCGCAC | ||||
| tetE | GGTATTACGGGAGTTTGTTGG | 60 | 199 | [23] |
| AATACAACACCCACACTACGC | ||||
| ermB | GAAAAGGTACTCAACCAAATA | 55 | 639 | [24] |
| AGTAACGGTACTTAAATTGTTTAC | ||||
| qnrS | ACGACATTCGTCAACTGCAA | 55 | 417 | [25] |
| TAAATTGGCACCCTGTAGGC |
