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Cytotoxicity of sixteen flavonoids on Crandell–Rees feline kidney cells at 24 h post-infection. Crandell–Rees feline kidney cells treated with each flavonoid at 100 μM were stained with 0.5% crystal violet to reveal viable cells (upper panels). The scale bar is 100 μm. The cytotoxicity was measured using MTS assay (lower panels). The 50% cytotoxicity concentrations values were calculated using non-linear regression analysis. Bars present mean and standard deviation values.
