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. 2023 Mar 26;16(3):618–630. doi: 10.14202/vetworld.2023.618-630

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Copyright: © Triratapiban, et al.

Open Access. This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Figure-4 — (a) Antiviral activity assay of isoginkgetin and luteolin. Both compounds could exert their inhibitory effects after feline infectious peritonitis virus (FIPV) infection for 2 h before the treatment, when compared to the positive drug control (GC376). 0.1% dimethyl sulfoxide (DMSO) in Crandell–Rees feline kidney (CRFK) cells was included as a negative control. The scale bar is 100 μm. (b and c) Viral loads of infected CRFK cells treated with isoginkgetin and (d and e) luteolin with a dose-dependent manner were quantified by reverse transcription-quantitative polymerase chain reaction. (b and d) The data were presented as quantification of viral copy numbers and (c and e) percentage of viral reduction, when compared to those of virus treated with 0.1% DMSO group. Differences between the flavonoid-treated and the FIPV-infected CRFK cells in a dose-dependent manner compared to virus control were assessed using analysis of variance (***p < 0.001).