Skip to main content
. 2023 Apr 8;14:1994. doi: 10.1038/s41467-023-37517-6

Fig. 3. Artificial elevation of systemic eNAMPT activity increases locomotor activity and energy expenditure.

Fig. 3

a Schematic diagram of the intraperitoneal (i.p.) NAMPT/NMN injection study. ZT: zeitgeber time. b The mediobasal hypothalamus (MBH) and liver NAD+ levels at ZT6 1 h after i.p. administration of NAMPT (0.3 mg/kg) and NMN (120 mg/kg) (MBH, n = 5; Liver, n = 3 mice). One-way ANOVA followed by Fisher’s LSD test. n.s.: not significant. c, d Effects of i.p. administration of NAMPT and NMN on daytime locomotor activity (LMA) and energy expenditure (EE) in mice (n = 5 mice). LMA and EE were monitored for 4 h after injections. One-way ANOVA with Fisher’s LSD test. e Schematic diagram of the intracerebroventricular (i.c.v.) NAMPT injection study. f The MBH NAD+ levels at ZT6 1 h after i.c.v. NAMPT administration (n = 4 mice). Unpaired t-test (two-sided). g, h Effects of i.c.v. administration of NAMPT (100 ng) on LMA (g) and EE (h) (n = 4 mice). Two-way ANOVA followed by Fisher’s LSD test. Two independent replicates were performed for all studies. Data are presented as mean ± SEM.