Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2023 Apr 8;14:1979. doi: 10.1038/s41467-023-37712-5

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Fig. 2 — a Genomic localization of various transcriptional regulators in HEK293T cells. ChIP-seq analysis of the chromatin of HEK293T cells for the indicated proteins. The average ChIP signal distribution of the indicated proteins around the transcriptional stat sites (TSSs) is shown for the ENL-target genes (red) or all genes (black). ChIP-seq tags of ENL and its input chromatin at all genes were clustered into a 2-kb bin (0 to +2 kb from the TSS) and the genes with the ENL ChIP/input ratio ≥2 were defined as ENL target genes. b Protein expression of MOZ and DOT1L and histone modification levels. WB of the of HEK293T cells was performed in two technical replicates of the parental clone and two biological replicates of MOZ- or DOT1L-knockout clones each. c HOXA gene expression in MOZ and DOT1L-deficient cells. RT-qPCR was performed on the HEK293T clones shown in b (mean of two technical replicates). d Localization of ENL and DOT1L in MOZ-deficient cells. ChIP-seq analysis was performed on MOZ knockout HEK293T cell line and its parental clone. ChIP-signals were visualized using the Integrative genome browser (IGV, Broad Institute). e Relative ChIP signals of ENL between a MOZ-deficient cell line and its parental clone. ENL ChIP signals of the dMOZ#10 clone in the bins of 0–1 kb of the top 100 ENL-bound genes of its parental clone were subdivided by those of the parental clone. HOXA6 is highlighted in red. f Relative abundance of DOT1L ChIP signals of the top 100 DOT1L-bound genes in a MOZ-deficient clone is shown as in e. g Localization of MOZ, DOT1L, and ENL. ChIP-qPCR was performed on two technical/biological replicates of each genotype for the indicated gene loci using qPCR probes designed for the pre-TSS ( − 1 to −0.5 kb of TSS) and post-TSS regions (+1 to +1.5 kb of TSS) of each gene. ChIP signals are expressed as a percentage of the input with error bars (mean of two technical/biological replicates). Source data are provided as a Source Data file.