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. 2023 Apr 8;14:1979. doi: 10.1038/s41467-023-37712-5

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 3 — a Structures required for NES-ENL-mediated transformation. Various ENL constructs were examined for the transformation of myeloid progenitors as in Fig. 1a. b Protein expression of various ENL constructs in stably transduced HEK293T cells. FLAG-tagged (denoted as f) gene constructs were lentivirally transduced to HEK293T cells and selected for puromycin resistance. WB of the whole-cell extracts was performed using anti-FLAG antibodies. c HOX gene expression in HEK293T cells stably expressing ENL mutants. RT-qPCR was performed on the cells presented in c (mean of two technical replicates). Multiple comparisons were performed using a one-way ANOVA. d Protein interaction of NES-ENL in the chromatin fraction. IP-WB of the chromatin fraction of HEK293T cells stably expressing various FLAG-tagged ENL constructs was performed using specific antibodies for the indicated proteins. e Localization of the various ENL constructs and CALM-AF10. ChIP-qPCR for the FLAG-tagged proteins, MOZ, and DOT1L were performed as in Fig. 2d. (mean of two technical replicates). Source data are provided as a Source Data file.