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. Author manuscript; available in PMC: 2023 Apr 9.
Published in final edited form as: Biochemistry. 2023 Feb 22;62(5):1082–1092. doi: 10.1021/acs.biochem.3c00021

Figure 2.

Figure 2.

Decay kinetics of the high-valent state in MbnH. (A) Decay kinetics of the putative bis-Fe(IV) state monitored in the nIR region at room temperature upon the addition of 10–200 μM H2O2 to 20 μM MbnH. A global fit of the data to the proposed model for decay is shown. Inset shows the absorbance traces over time for the addition of 200 μM H2O2 to MbnH. (B) Decay of the nIR feature as a function of temperature upon mixing 200 μM H2O2 with 20 μM MbnH. The data were fit to a single exponential to extract kobs for the bis-Fe(IV) decay. The inset shows decay rates as a function of temperature with data fit to the Arrhenius equation.