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. Author manuscript; available in PMC: 2024 Jan 1.
Published in final edited form as: Methods Mol Biol. 2023;2651:251–275. doi: 10.1007/978-1-0716-3084-6_18

Figure 1. Pipeline for the biophysical characterization of Zα-dependent A-Z junction adoption in RNAs.

Figure 1.

An overview of the steps required for characterization of A-Z junction adoption upon binding to Zα by Circular Dichroism (CD), Isothermal Titration Calorimetry (ITC), Analytical Ultracentrifugation (AUC), and Nuclear Magnetic Resonance (NMR) is shown.