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. Author manuscript; available in PMC: 2024 Jan 1.
Published in final edited form as: Methods Mol Biol. 2023;2651:251–275. doi: 10.1007/978-1-0716-3084-6_18

Figure 2. Circular Dichroism to investigate A-Z junction adoption and determine the extent of Z-RNA formation.

Figure 2.

(a) CD spectra of the (CpG)3, an A-Z junction positive control, and an (ApU)6 negative control RNA in the absence of protein (black), with 6 M sodium perchlorate (dotted black line), and with a molar ratio of 1:6 RNA:Zα (red) at which binding is saturated. (b) Extent of Z-RNA (EZ) scores quantifying the extent of Z-conformation for the (ApU)6, A-Z junction control, AluSx1Jo, and (CpG)3 RNAs.