Table 1.

Example setup for circular dichroism titration of RNA with Zα.

CD experiment / titration point	RNA stock (1 mM)	Zα stock (2 mM)	Buffer (20 mM potassium phosphate (pH 6.4), 25 mM NaCl, 0.5 mM EDTA, 1mM DTT)	Sodium perchlorate (8 M)
Buffer control			175 μL
free RNA	8.75 μL		166.25 μL
free Zα control		26.25 μL	148.75 μL
Z-RNA control	8.75 μL		35 μL	131.25 μL
6:1 RNA:Zα	8.75 μL	0.73 μL	165.52 μL
1:1 RNA:Zα	8.75 μL	4.38 μL	153.12 μL
1:6 RNA:Zα	8.75 μL	26.25 μL	140 μL