(A) NOD mice were treated with either vehicle or MOTS-c daily (0.5 mg/kg/day; IP) for 18 weeks of age. Then, NOD splenocytes (n=5) and CD4+ T cells were assessed for (B, D) glycolysis after glucose stimulation (n=10) and (C, E) respiratory capacity (n=10); two-tailed t-test (B, C, D, E), error bars are S.E.M. *p<0.05, **p<0.01, ****p<0.0001.
(F) Jurkat cells were overexpressed with empty vector, MOTS-cWT, and MOTS-cMut. These cells were assessed for (G) glycolysis after glucose stimulation (n=5) and (H) respiratory capacity (n=5); one-way ANOVA (G, H), error bars are S.E.M. **p<0.01, ****p<0.0001.