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. 2023 Mar 27;14:1138582. doi: 10.3389/fgene.2023.1138582

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Copyright © 2023 Munro, Holmes, Moore, Carlile, Payne, Tyson, Williams, Alder, Snell, Nebbia, Santos and Loose.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Genome recovery throughout all 13 runs. Blue line (line of best fit), Orange line (x = y). (A) Compares the predicted genome recovery based on 1 hours sequencing (X-axis) with the actual coverage seen at the end of the run (Y-axis). Predicted recovery is the proportion of amplicons in a sample expected to reach 20× coverage. Actual coverage is the proportion of non N bases in the consensus genomes obtained at the end of the run (Full Run). (B) Compares the same predicted genome recovery with the actual coverage observed at the Run Complete time as defined by minoTour. (C) Compares the actual coverages reported in A (Full Run) and B (Run Complete). (D) Is the same as C but ignores those runs where the Run Complete time is the same as the Full Run time.