Table 4.

Insulin values (μIU/ml) in triplicate at each level showing individual values, mean values, range, standard deviation (SD), CV% (coefficient of variation percent), percent bias (bias%), and total observed error (TEo%).

Observed concentrations and range (μIU/ml)	Mean (μIU/ml)	Expected concentration (μIU/ml)	Mean (μIU/ml)	SD (%)	CV (%)	Bias (%)	TEo (%)
315	304.7	309	304.7	9.6	3.15%	1.39%	7.7%
303
296
139	145.3	154.5	145.3	5.7	3.91%	5.95%	13.8%
150
147
77.4	75.7	77.3	75.7	3.6	4.76%	2.07%	11.6%
71.6
78.2
40.1	40.1	38.6	40.1	2.0	4.86%	3.89%	13.6%
38.2
42.1
23.7	22.1	19.3	22.1	1.6	7.26%	14.51%	29.0%
20.5
22
11	11.4	9.7	11.4	0.7	5.82%	17.53%	29.2%
12.2
11.1
5.49	5.8	4.8	5.8	0.7	12.46%	20.83%	45.7%
5.29
6.63
3.41	4.3	2.4	4.3	1.3	31.25%	79.17%	141.7%
3.61
5.82
2.96	2.5	1.2	2.5	0.4	16.22%	108.33%	140.8%
2.21
2.32

TEo% values of <25% were considered acceptable at insulin values of 40 μIU/ml and higher. TEo% values for insulin were >25% in insulin samples <40 μIU/ml. Observed total error (TEO; %) was calculated as |bias (%)| + 2 CV.