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. 2023 Mar 27;14:1146612. doi: 10.3389/fimmu.2023.1146612

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Copyright © 2023 Liu, Zhou, Tu, Yao, Li and Yang

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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ALA inhibited NETs-induced pyroptosis of macrophages in vitro. The bone marrow-derived macrophages (BMDMs) were pretreated with different concentrations of ALA (0, 50, 100, 250, 500 nM/ml), then incubated with NETs for 24 h. (A–D) Immunoblot analysis of pyroptosis-related proteins (Cl-caspase-1, Cl-GSDMD, ASC) expression in BMDMs; (E) Level of IL-1β in cell supernatant was determined by ELISA; (F, G) Immunofluorescence assay for cell death in BMDMs after treatment with NETs (6 μg/ml, 24h); (H, I) Images are representative of three independent experiments. Data were represented as mean ± S.D. (n = 3). *P < 0.05, **P < 0.01, ***P<0.001, **** P < 0.0001.