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. 2023 Mar 27;14:1146612. doi: 10.3389/fimmu.2023.1146612

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Copyright © 2023 Liu, Zhou, Tu, Yao, Li and Yang

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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NETs induce macrophage pyroptosis by activating the Pyrin inflammasome. (A) Immunofluorescence images of bone marrow-derived macrophages (BMDMs) after treatment with NETs (6 μg/ml, 24h). Arrowheads indicate the ASC/Pyrin speck. Images are representative of three independent experiments. (B–D) Immunoblot analysis of pyroptosis-related proteins (Cl-caspase-1, Cl-GSDMD, ASC) expression in the BMDMs from wild-type or Mefv (Pyrin) ^-/- mice after treated with NETs (6 μg/ml, 24 h); (E) Level of inflammatory cytokine IL-1β in cell supernatant was detected by ELISA. Data were represented as mean ± S.D. (n = 3). “ns” represents no significant difference, *P < 0.05, **P < 0.01, ***P<0.001, ****P < 0.0001.