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. 2023 Mar 27;14:1146612. doi: 10.3389/fimmu.2023.1146612

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Copyright © 2023 Liu, Zhou, Tu, Yao, Li and Yang

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Pyrin inflammasome-driven macrophage pyroptosis contributed to sepsis-induced ALI/ARDS. WT or Mefv (Pyrin) ^-/- mice were subjected to ALI/ARDS by intratracheal instillation of LPS (10 mg/kg). At 24 h after LPS instillation, (A) haematoxylin and eosin (HE) staining lung tissue sections were examined histologically; (B) lung injury pathology score was assessed; (C) total protein concentration in BALF was determined by a BCA protein assay kit; (D) level of inflammatory cytokine IL-1β in BALF was detected by ELISA; (E-H) expression of pyroptosis-related proteins (Cl-caspase-1, Cl-GSDMD, ASC) in lung tissue was analyzed by western blotting. Data were represented as mean ± S.D. (n = 3). “ns” represents no significant difference, *P < 0.05, **P < 0.01, ***P<0.001, **** P < 0.0001.