Item No	Recommendation	Page No
Title and abstract	1	(a) Indicate the study's design with a commonly used term in the title or the abstract	1
		(b) Provide in the abstract an informative and balanced summary of what was done and what was found	4–5
Introduction
Background/rationale	2	Explain the scientific background and rationale for the investigation being reported	6–7
Objectives	3	State specific objectives, including any prespecified hypotheses	7
Methods
Study design	4	Present key elements of study design early in the paper	8
Setting	5	Describe the setting, locations, and relevant dates, including periods of recruitment, exposure, follow‐up, and data collection	8–9
Participants	6	(a) Cohort study—Give the eligibility criteria, and the sources and methods of selection of participants. Describe methods of follow‐up	8–9
		Case‐control study—Give the eligibility criteria, and the sources and methods of case ascertainment and control selection. Give the rationale for the choice of cases and controls
		Cross‐sectional study—Give the eligibility criteria, and the sources and methods of selection of participants
		(b) Cohort study—For matched studies, give matching criteria and number of exposed and unexposed	8–9
		Case‐control study—For matched studies, give matching criteria and the number of controls per case
Variables	7	Clearly define all outcomes, exposures, predictors, potential confounders, and effect modifiers. Give diagnostic criteria, if applicable	8–9
Data sources/measurement	8*	For each variable of interest, give sources of data and details of methods of assessment (measurement). Describe the comparability of assessment methods if there is more than one group	8–9
Bias	9	Describe any efforts to address potential sources of bias	8–10
Study size	10	Explain how the study size was arrived at	8–10
Quantitative variables	11	Explain how quantitative variables were handled in the analyses. If applicable, describe which groupings were chosen and why	10
Statistical methods	12	(a) Describe all statistical methods, including those used to control for confounding	10
		(b) Describe any methods used to examine subgroups and interactions	10
		(c) Explain how missing data were addressed	10
		(d) Cohort study—If applicable, explain how loss to follow‐up was addressed	NA
		Case‐control study—If applicable, explains how matching of cases and controls was addressed
		Cross‐sectional study—If applicable, describes analytical methods taking into account the sampling strategy
		(e) Describe any sensitivity analyses	10
Results
Participants	13*	(a) Report numbers of individuals at each stage of study, e.g., numbers potentially eligible, examined for eligibility, confirmed eligible, included in the study, completing follow‐up, and analysed	11
		(b) Give reasons for non‐participation at each stage	11
		(c) Consider the use of a flow diagram	NA
Descriptive data	14*	(a) Give characteristics of study participants (eg demographic, clinical, social) and information on exposures and potential confounders	11
		(b) Indicate the number of participants with missing data for each variable of interest	11
		(c) Cohort study—Summarise follow‐up time (e.g. average and total amount)	11
Outcome data	15*	Cohort study—Report numbers of outcome events or summary measures over time	NA
		Case‐control study—Report numbers in each exposure category or summary measures of exposure	11–13
		Cross‐sectional study—Report numbers of outcome events or summary measures	NA
Main results	16	(a) Give unadjusted estimates and, if applicable, confounder‐adjusted estimates and their precision (e.g. 95% confidence interval). Make clear which confounders were adjusted for and why they were included	11–13
		(b) Report category boundaries when continuous variables were categorized	11–13
		(c) If relevant, consider translating estimates of relative risk into absolute risk of a meaningful time period	NA
Other analyses	17	Report other analyses conducted, analyses of subgroups and interactions, and sensitivity analyses	11–13
Discussion
Key results	18	Summarise key results with reference to study objectives	14–15
Limitations	19	Discuss the limitations of the study, taking into account sources of potential bias or imprecision. Discuss both direction and magnitude of any potential bias	17
Interpretation	20	Give a cautious overall interpretation of results considering objectives, limitations, multiplicity of analyses, results from similar studies, and other relevant evidence	14–17
Generalisability	21	Discuss the generalisability (external validity) of the study results	16–17
Other information
Funding	22	Give the source of funding and the role of the funders for the present study and, if applicable, for the original study on which the present article is based	NA

Note: An Explanation and Elaboration article discusses each checklist item and gives methodological background and published examples of transparent reporting. The STROBE checklist is best used in conjunction with this article (freely available on the Web sites of PLoS Medicine at http://www.plosmedicine.org/, Annals of Internal Medicine at http://www.annals.org/, and Epidemiology at http://www.epidem.com/). Information on the STROBE Initiative is available at www.strobe-statement.org.

*Give information separately for cases and controls in case‐control studies and, if applicable, for exposed and unexposed groups in cohort and cross‐sectional studies.