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Schematic description of the LPS flipping assay. Mfsd2a proteoliposomes encapsulating PSVue550 are incubated in the presence of LPS and NaCl. Sodium-coupled LPS flipping catalyzed by Mfsd2a, expose the phosphoserine headgroup of LPS at the inner leaflet of the bilayer. Binding of PSVue550 to LPS headgroups results in fluorescence emission at 610 nm.
