Figure 3.

NEDD4L knockdown alleviated granulosa cell ferroptosis. (A and B) The mRNA and protein level of NEDD4L in DHT-induced GCs was analyzed by qRT-PCR and western blot with or without si-NEDD4L treatment (n = 3). **P < 0.01, ***P < 0.001. (C) The cell viability of DHT-induced GCs was analyzed by the CCK-8 assay after treatment with or without si-NEDD4L (n = 3). **P < 0.01. (D) The cell viability of DHT-induced GCs was analyzed by FDA staining after treatment with or without si-NEDD4L (n = 3). *P < 0.05, **P < 0.01. (E–H) the level of Fe²⁺, the content of MDA, the level of ROS, and the content of GSH in DHT-induced GCs were assessed by the ELISA assay after treatment with or without si-NEDD4L (n = 3). *P < 0.05, **P < 0.01.