TABLE 1.
Specifications of the four used fluorophores.
| Fluorophore | Excitation source | QE | Extinction coefficient (M−1 cm−1) | F/P ratio | Brightness (M−1 cm−1) | Reference number |
|---|---|---|---|---|---|---|
| APC | Red | 0.68 | 700,000 | 1 | 4.8 × 105 | [15, 16, 17] |
| BV421 | Violet/blue | 0.69 | 2,500,000 | ‐ | 1.7 × 106 a | [17] |
| FITC | Green | 0.92 | 72,000 | 2.3 | 1.5 × 105 | [18] |
| PE | Blue‐green/yellow | 0.82 | 1,960,000 | 1 | 1.6 × 106 | [15, 17] |
Note: Excitation sources, quantum efficiency (QE), extinction coefficient, fluorophore to protein (F/P) ratio, and brightness of allophycocyanin (APC), Brilliant Violet 421 (BV421), fluorescein isothiocyanate (FITC), and phycoerythrin (PE). The F/P ratio and relative quantum yield for the four different fluorophores are either requested from the manufacturer or cited from literature.
a
Assuming that the F/P ratio is 1.