Fig. 4.

Ginger extract attenuated lipids accumulation in 3T3-L1 pre-adipocyte during adipocyte differentiation. 3T3-L1 pre-adipocyte cells were treated with different ginger extracts treatments (0, 25, and 50 μg/mL). (a) Changes observed in adipocyte differentiation on day 7 of incubation are presented with Oil Red O staining. Cell images were captured at 200× magnification. (b) Cells stained with Oil Red O extracted in propanol, and the optical density was recorded using a microplate reader. Each experiment was performed at least four times, and the individual results are expressed as mean ± SD. One-way ANOVA and Tukey’s HSD post hoc were performed using SPSS Statistics 20 software to determine differences among group means. Within groups, statistically significant difference was represented by different letters, indicating that groups were significantly different at P < 0.05. Treatments with the same letter were not significantly different from each other.