Fig. 1. Steroid affinity test on BaCYP106A6.
(A) Type I shift spectra and Kd values using progesterone and 4- androstenedione. A total of six substrates were tested for BaCYP106A6 binding. Progesterone and androstenedione showed binding affinity, whereas corticosterone, cortisol, dexamethasone, and prednisolone did not show consistent data for affinity calculation. (B) Steroids with a BaCYP106A6-associated conversion rate > 20% were considered active steroids. (C) Steroids with a BaCYP106A6-associated conversion rate < 20% were considered less active steroids. The conversion rate of steroids was analyzed by HPLC after 15 min incubation at 20°C.