Skip to main content
. 2023 Jan 6;33(3):319–328. doi: 10.4014/jmb.2212.12026

Fig. 5. Transcriptional expression of secretory aspartyl proteases in M. restricta and M. globosa at lower pH.

Fig. 5

A. The pH of the media of M. restricta axenic culture (M.R), M. restricta co-cultured with S. epidermidis (M.R+S.E), and M. restricta co-cultured with S. aureus (M.R+S.A) before and after 12 h of incubation. The initial pH of the media was 6.5–6.7. B. Relative expression levels of genes encoding aspartyl proteases in M. restricta in media at pH 6.5 (M.R at pH 6.5), pH 5.5 (M.R at pH 5.5), and pH 5.0 (M.R at pH 5.0). Expression levels were normalized to those of actin (MRET_1518) and compared to the expression levels observed in M.R at pH 6.5. C. Relative expression levels of MGL_3328 and MGL_1932 in M. globosa (M.G) axenic culture, M. globosa co-cultured with S. epidermidis (M.G+S.E), and M. globosa co-cultured with S. aureus (M.G+S.A). Gene expression levels were normalized to those of actin (MGL_1986) and compared to the expression levels observed in M.G axenic culture. D. Relative expression of genes encoding aspartyl proteases in M. globosa at pH 6.5 (M.G at pH 6.5), pH 5.5 (M.G at pH 5.5), and pH 5.0 (M.G at pH 5.0). Gene expression levels were normalized to those of actin (MGL_1986) and compared to the expression levels observed in M.G at pH 6.5. *, p < 0.1; **, p < 0.01; ***, p < 0.001.