Fig. 6.

Physiological analysis of D. radiodurans R-M deletion strains. (A) Growth curves of D. radiodurans WT and ∆RM knockout strains grown in liquid TGY media for 17 h. Each data point represents the mean of 3 biological and 2 technical replicates, with error bars representing standard error of the mean. The doubling time for each strain is reported (in minutes) in the legend and represents the mean value of the 3 biological and 2 technical replicates ± the standard deviation. (B) Transformation efficiency reported as CFU μg⁻¹ DNA for heat shock transformation of the pRAD1 plasmid into D. radiodurans WT and ∆RM knockout strains. Transformants were selected on TGY media supplemented with chloramphenicol (3 μg ml⁻¹), and 850 ng of plasmid DNA was used for each transformation. The data presented is the mean of 3 biological replicates with each dot representing one replicate and error bars representing standard error of the mean. A 2-sample, 2-tailed Student t test was used to compare WT (control) to each ∆RM knockout strain: *P < 0.05.