Figure 1.

Tacrolimus induces kidney damage in the form of renal fibrosis in mice. To establish that our in vivo model recapitulates the morphological features of calcineurin inhibitor-induced nephropathy, wild-type mice were treated with either 10 mg/kg tacrolimus or vehicle (ethanol/DMSO/saline) for 21 days (A). Tacrolimus-induced histological lesions were assessed by hematoxylin and eosin (H&E) staining (B, box). Extracellular matrix accumulation (collagen) was assessed by both Masson trichrome staining (blue; C, arrows) and picrosirius red staining (D). Representative images shown of 8 animals per group.