Figure 4.

A combination of oligo-FISH and EdU labeled SCEs endorse the helical chromatin organization. Metaphase chromosome spread (top) showing all barley chromosomes after EdU and oligo-FISH labeling. In both homologous oligo-FISH painted 5HL regions two SCEs occurred (bottom). The left exchanged segment does not span the entire chromatid width, but the right one does (insets in the merged images). The SCEs are present at the transition from the Rhea region to the not labeled Moa (left, red arrow) and Ostrich (right, white arrow) regions. The heights of the exchanged segments, oligo-FISH probe and EdU-free regions are similar (∼380–450 nm).