Table 1.

Spectral properties of the dyes in complexes with the aptamers

					Φ_Mango-dye (r.u.)b		Brightness_Mango-dye (M−1cm−1)b,c
Dye	Free dye abs. max (nm)	Free dyea ϵ (M−1cm−1)	Mango-dye λex (nm)	Mango-dye λem (nm)	Mango II	Mango IV	Mango II	Mango IV
TO1-biotin	495	77500	505	540	0.39	0.43	29200	32200
4a	495	77500	505	540	0.42	0.32	29400	19900
4b	535	62400	560	615	0.44	0.29	26200	15900
4с	580	101100	595	640	0.07	0.05	7100	5400
4d	580	51300	595	650	0.14	0.10	6400	4700

^aMolar extinction at absorbance maximum of the free dye.

^bConditions: 5 μM Mango aptamer, 5 μM dye, 10 mM Tris–HCl, pH 7.5, and 140 mM КСl.

^cBrightness = molar extinction (ϵ) at complex max*Ф.