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. 2023 Feb 2;51(6):2655–2670. doi: 10.1093/nar/gkad043

Figure 1.

Figure 1.

SMAD3 promotes the expression of AR and AR targets. (A) Volcano plot showing the differentially expressed genes between control and SMAD3-KD Rv1 cells in the RNA-seq analysis. (B) GO analysis of the downregulated genes after SMAD3 KD showing enrichment of the AR signaling pathway. (C) BART analysis of the downregulated genes after SMAD3 KD. AR is predicted to be a top transcription factor altered after SMAD3 KD. (D, E) Real-time RT-PCR results showing the reduced mRNA levels of AR, AR-V7 and example AR targets in the SMAD3-KD Rv1 (D) or C4-2 (E) cells. Quantification was presented as mean ± SD (n = 3), and t test was used for statistical analysis (***P < 0.001). (F) Western blots showing the reduced level of AR and AR-V7 in the SMAD3-KD Rv1 or C4-2 cells. (G) Real-time RT-PCR results showing the reduced mRNA level of AR and AR-V7 in the SMAD3-KD LN95 cells. Quantification was presented as mean ± SD (n = 3), and t test was used for statistical analysis (**P < 0.01; ***P < 0.001). (H) Western blots showing the reduced level of AR and AR-V7 in the SMAD3-KD LN95 cells.