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. 2023 Feb 2;51(6):2655–2670. doi: 10.1093/nar/gkad043

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© The Author(s) 2023. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 2. — SMAD4 or SMAD2 has little or no effect on the expression of AR and AR targets. (A, B) Volcano plot of RNA-seq results showing the differentially expressed genes upon KD of SMAD2 (A) or SMAD4 (B) in Rv1 cells. (C) Heatmap showing the altered level of AR and classic AR target genes in the RNA-seq analysis of SMAD3-KD, SMAD4-KD or SMAD2-KD Rv1 cells. The FPKM value of RNA-seq results was log₂ transformed and used for the heatmap preparation using the pheatmap package in R. (D) GO analysis of the downregulated genes after SMAD2 KD. (E) BART analysis of the downregulated genes after SMAD2 KD to predict the altered transcription factors. (F) GO analysis of the downregulated genes after SMAD4 KD. (G) BART analysis of the downregulated genes after SMAD4 KD to predict the altered transcription factors.