Figure 6.
SMAD3 KD decreases the global ChIP-seq signal of AR. (A) Alteration of AR ChIP-seq peaks after SMAD3 KD in Rv1 cells. Cut&Run ChIP-seq studies were performed on Rv1 cells (control and SMAD3 KD) using AR antibodies. The alteration of AR peaks is shown in the Venn diagram including the control-enriched peaks, common peaks and SMAD3-KD-enriched peaks. (B) The genome distribution of AR peaks in control (n = 13073) and SMAD3-KD (n = 3997) Rv1 cells. (C) Heatmap showing the AR ChIP-seq peaks in control and SMAD3-KD Rv1 cells including the control-enriched peaks, common peaks and SMAD3-KD-enriched peaks. (D) Example signal track image showing the AR peak at KLK3 enhancer, KLK2 promoter or NKX3-1 promoter (indicated with an arrow) in control and SMAD3-KD Rv1 cells.
